Abstract: This work aims to provide a strategy for rapidly screening food raw materials of bovine origin for the presence of the most frequent O-serogroups of Shiga toxin-encoding Escherichia coli (STEC) involved in food poisoning outbreaks. The prevalence of highly pathogenic serogroups of STEC was surveyed in 25 g portions of minced meat and raw milk using PCR-ELISA and multiplex real-time PCR assays. The prevalence of STEC in raw milk (n = 205) and meat samples (n = 300) was 21% and 15%, respectively. Contamination by the main pathogenic E. coli O-ser...
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Topics:
Food science
Microbiology