BIP! Finder - Reference Gene Identification for Reverse Transcription-Quantitative Polymerase Chain Reaction Analysis in an Ischemic Wound-Healing Model

2013 • Reference Gene Identification for Reverse Transcription-Quantitative Polymerase Chain Reaction Analysis in an Ischemic Wound-Healing Model

Authors: Elizabeth D. Ruedrich; Kath M. Bogie; Mary K. Henzel; Bryan S. Hausman

Venue: Journal of Biomolecular Techniques : JBT

Type: Publication

Abstract: Reference genes are often used in RT-quantitative PCR (qPCR) analysis to normalize gene expression levels to a gene that is expressed stably across study groups. They ultimately serve as a control in RT-qPCR analysis, producing more accurate interpretation of results. Whereas many reference genes have been used in various wound-healing studies, the most stable reference gene for ischemic wound-healing analysis has yet to be identified. The goal of this study was to determine systematically the most stable reference gene for studying gene expres... (read more)

Impact: 3.2091387E-9 2.9854916E-9 10 3 / Attention: 0 7

Topics: Computational biology Bioinformatics

DOI: 10.7171/jbt.13-2404-003

External links: Crossref OpenAIRE

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