BIP! Finder - Comparison of In Situ Hybridization, Immunohistochemistry, and Reverse Transcription–Droplet Digital Polymerase Chain Reaction for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Testing in Tissue

2021 • Comparison of In Situ Hybridization, Immunohistochemistry, and Reverse Transcription–Droplet Digital Polymerase Chain Reaction for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Testing in Tissue

Authors: Anja C. Roden, Julie A. Vrana, Justin W. Koepplin, Angela E. Hudson, Andrew P. Norgan, Garrett Jenkinson, Satoko Yamaoka, Hideki Ebihara, Robert Monroe, Matthias J. Szabolcs, Ramanath Majumdar, Ann M. Moyer, Joaquín J. García, Benjamin R. Kipp

Venue: Archives of Pathology & Laboratory Medicine

Type: Article

Abstract: Small case series have evaluated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection in formalin-fixed, paraffin-embedded tissue using reverse transcription–polymerase chain reaction, immunohistochemistry (IHC), and/or RNA in situ hybridization (RNAish).To compare droplet digital polymerase chain reaction, IHC, and RNAish to detect SARS-CoV-2 in formalin-fixed, paraffin-embedded tissue in a large series of lung specimens from coronavirus disease 2019 (COVID-19) patients.Droplet digital polymerase chain reaction and RNAish u... (read more)

Topics: Pathology Molecular biology

DOI: 10.5858/arpa.2021-0008-sa

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External links: Crossref , OpenAIRE

References (27) Citations (14) BibTex PDF

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