Authors: Corman, Victor M., Müller, Marcel A., Costabel, Ulrich, Timm, Jörg, Binger, Tabea, Meyer, Benjamin, Kreher, Petra, Lattwein, Erik, Eschbach-Bludau, Monika, Nitsche, Andreas, Bleicker, Tobias, Landt, Olfert, Schweiger, Brunhilde, Drexler, Jan F., Osterhaus, Albert, Haagmans, Bart L., Dittmer, Ulf, Bonin, Frank, Wolff, Thorsten, Drosten, Christian
Venue: Eurosurveillance
Type: Publication
Abstract: We present a rigorously validated and highly sensitive confirmatory real-time RT-PCR assay (1A assay) that can be used in combination with the previously reported upE assay. Two additional RT-PCR assays for sequencing are described, targeting the RdRp gene (RdRpSeq assay) and N gene (NSeq assay), where an insertion/deletion polymorphism might exist among different hCoV-EMC strains. Finally, a simplified and biologically safe protocol for detection of antibody response by immunofluorescence microscopy was developed using convalescent patient ser...
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Topics: 
Virology
Molecular biology
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