BIP! Finder - Direct Detection and Characterization of Shiga Toxigenic Escherichia coli by Multiplex PCR for stx 1 , stx 2 , eae , ehxA , and saa

2002 • Direct Detection and Characterization of Shiga Toxigenic Escherichia coli by Multiplex PCR for stx 1 , stx 2 , eae , ehxA , and saa

Authors: Adrienne W. Paton, James C. Paton

Venue: Journal of Clinical Microbiology

Type: Publication

Abstract: ABSTRACT We recently described a novel megaplasmid-encoded adhesin produced by certain Shiga toxigenic Escherichia coli (STEC) strains that lack the locus for enterocyte effacement (LEE) pathogenicity island. This adhesin, designated Saa (STEC autoagglutinating adhesin), may be a marker for a subset of LEE-negative STEC strains capable of causing severe gastrointestinal and systemic diseases in humans. In this study, we developed a pentavalent PCR assay for the detection of saa as well as other proven and putative STEC virulence genes ( stx 1 ,... (read more)

Topics: Microbiology Virology

DOI: 10.1128/jcm.40.1.271-274.2002

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External links: Crossref OpenAIRE

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