Abstract: Aims: To evaluate the occurrence and abundance of phages that carry the stx1 and stx2 gene in water samples of different quality. Methods and Results: Phages growing on the Shiga toxin-negative Escherichia coli O157:H7 (ATCC 43888) strain were enumerated by a plaque assay in concentrated raw and treated waste water samples and river water samples. Plaques were investigated for the presence of stx1 and stx2 genes by a multiplex/nested PCR procedure. An overall number of 805 plaques were tested for the presence of stx-carrying phages. Stx genes c...
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Topics: 
Microbiology