Authors: Ekeberg, Tomas; Kauffmann, Martin; Abergel, Chantal; Maia, Filipe; Seltzer, Virginie; Claverie, Jean-Michel; Hantke, Max; Jönsson, Olof; Nettelblad, Carl; van Der Schot, Gijs; Liang, Mengning; Deponte, Daniel; Barty, Anton; Seibert, M Marvin; Iwan, Bianca; Andersson, Inger; Loh, N Duane; Martin, Andrew; Chapman, Henry; Bostedt, Christoph; Bozek, John; Ferguson, Ken; Krzywinski, Jacek; Epp, Sascha; Rolles, Daniel; Rudenko, Artem; Hartmann, Robert; Kimmel, Nils; Hajdu, Janos; Maia, Filipe R. n. c.; Seibert, M. Marvin; Loh, N. Duane
Venue: Physical Review Letters
Type: Publication
Abstract: We present a proof-of-concept three-dimensional reconstruction of the giant mimivirus particle from experimentally measured diffraction patterns from an x-ray free-electron laser. Three-dimensional imaging requires the assembly of many two-dimensional patterns into an internally consistent Fourier volume. Since each particle is randomly oriented when exposed to the x-ray pulse, relative orientations have to be retrieved from the diffraction data alone. We achieve this with a modified version of the expand, maximize and compress algorithm and va...
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