Authors: Peter Møller, Amaya Azqueta, Elisa Boutet-Robinet, Gudrun Koppen, Stefano Bonassi, Mirta Milić, Goran Gajski, Solange Costa, João Paulo Teixeira, Cristiana Pereira, Maria Dusinska, Roger W. L. Godschalk, Gunnar Brunborg, Kristine B. Gutzkow, Lisa Giovannelli, Marcus S. Cooke, Elke Richling, Blanca Laffon, Vanessa Valdiglesias, Nurşen Başaran, Cristian Del Bo, Bojana Zegura, Matjaz Novak, Helga Stopper, Pavel Vodicka, Sona Vodenkova, Vanessa Moraes de Andrade, Monika Sramkova, Alena Gábelová, Andrew Collins, Sabine A. S. Langie

Venue: Nature Protocols

Type: Publication

Abstract: The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describin... (read more)

DOI: 10.1038/s41596-020-0398-1