Abstract: A large somatostatin-like polypeptide of apparent molecular weight 3000-4500 [4K somatostatin (SS)] was isolated from ovine hypothalamus. The polypeptide was obtained in the methionine sulfoxide form. Two microsequence analyses of 0.6 and 1.8 nmol of 4K SS were performed with a modified 890 C spinning cup sequencer. The sequencing data together with results of amino acid analysis and C-terminal end-group determination indicated that 4K SS was identical with somatostatin-28 (SS-28) isolated from procine upper small intestine and sequenced by Pra...
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