Authors: Pang, Bo, Xu, Jingyang, Liu, Yanming, Peng, Hanyong, Feng, Wei, Cao, Yiren, Wu, Jinjun, Xiao, Huyan, Pabbaraju, Kanti, Tipples, Graham, Joyce, Michael A., Saffran, Holly A., Tyrrell, D. Lorne, Zhang, Hongquan, Le, X. Chris

Venue: Analytical Chemistry

Type: Publication

Abstract: We have developed a single-tube assay for SARS-CoV-2 in patient samples. This assay combined advantages of reverse transcription (RT) loop-mediated isothermal amplification (LAMP) with clustered regularly interspaced short palindromic repeats (CRISPRs) and the CRISPR-associated (Cas) enzyme Cas12a. Our assay is able to detect SARS-CoV-2 in a single tube within 40 min, requiring only a single temperature control (62 °C). The RT-LAMP reagents were added to the sample vial, while CRISPR Cas12a reagents were deposited onto the lid of the vial. Aft... (read more)

DOI: 10.1021/acs.analchem.0c04047