2008 •
Structure of human argininosuccinate synthetase.
Authors:
Tobias Karlberg, R. Collins, Susanne van den Berg, A. Flores, Martin Hammarström, Martin Högbom, Lovisa Holmberg Schiavone, J. Uppenberg
Abstract:
Argininosuccinate synthetase catalyzes the transformation of citrulline and aspartate into argininosuccinate and pyrophosphate using the hydrolysis of ATP to AMP and pyrophosphate. This enzymatic process constitutes the rate-limiting step in both the urea and arginine–citrulline cycles. Previous studies have investigated the crystal structures of argininosuccinate synthetase from bacterial species. In this work, the first crystal structure of human argininosuccinate synthetase in complex with the substrates citrulline and aspartate is pre (...)
Argininosuccinate synthetase catalyzes the transformation of citrulline and aspartate into argininosuccinate and pyrophosphate using the hydrolysis of ATP to AMP and pyrophosphate. This enzymatic process constitutes the rate-limiting step in both the urea and arginine–citrulline cycles. Previous studies have investigated the crystal structures of argininosuccinate synthetase from bacterial species. In this work, the first crystal structure of human argininosuccinate synthetase in complex with the substrates citrulline and aspartate is presented. The human enzyme is compared with structures of argininosuccinate synthetase from bacteria. In addition, the structure also provides new insights into the function of the numerous clinical mutations identified in patients with type I citrullinaemia (also known as classic citrullinaemia). (Read More)
Tobias Karlberg, Ruairi Collins, Susanne van den Berg, Alex Flores, Martin Hammarström, Martin Hög (...)
Acta Crystallographica Section D Biological Crystallography ·
2008
Biochemistry |
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