2021 •
Supplementary Figure S6: Improved SARS-COV-2 PCR detection and genotyping with double-bubble primers
Authors:
Ailenberg, Menachem, Kapus, Andras, Rotstein, Ori D
Abstract:Efficiency of D-B primers in detecting SARS-COV-2 virus in nasopharyngeal swabs. cDNA of reverse-transcribed RNA extracted from nasopharyngeal swabs of patient S1 underwent 3 decimal dilutions and subjected to qPCR with SYBR green kit+ UDG and D-B primer mix #8.(A) amplification plot and insert efficiency plot of Ct vs log dilution. Slope= -3.2942 representing 101.27% amplification efficiency and R2= 0.9934. (B) melt curve of the amplicons depicted in panel A. Ct dilution values: 100- (...) Efficiency of D-B primers in detecting SARS-COV-2 virus in nasopharyngeal swabs. cDNA of reverse-transcribed RNA extracted from nasopharyngeal swabs of patient S1 underwent 3 decimal dilutions and subjected to qPCR with SYBR green kit+ UDG and D-B primer mix #8.(A) amplification plot and insert efficiency plot of Ct vs log dilution. Slope= -3.2942 representing 101.27% amplification efficiency and R2= 0.9934. (B) melt curve of the amplicons depicted in panel A. Ct dilution values: 100- 25.55, 10-1- 28.38, 10-2- 32.14, NTC- 39.48. qPCRwas performed under standard conditions.(Read More)
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