1999 •
Influence of spironolactone after prenatal exposion on male fertility of the rat
Authors:
Wittchen, Silke
Abstract:
Tree groups of pregnant femal rats were formed: a control group and two groups treated with spironolactone (twice 20mg/kg BW at an eight-hour interval on the 10th or 15th day of gestation). The stage of development of the offspring was evaluated on the basis of the following criteria: individual body weight from the 1st to 21st day of life, time of eye opening, time of eruption of teeth, time of fur development and - with male offspring - time of decline of the testes. A change in postnatal development caused by this substance was not observed. (...)
Tree groups of pregnant femal rats were formed: a control group and two groups treated with spironolactone (twice 20mg/kg BW at an eight-hour interval on the 10th or 15th day of gestation). The stage of development of the offspring was evaluated on the basis of the following criteria: individual body weight from the 1st to 21st day of life, time of eye opening, time of eruption of teeth, time of fur development and - with male offspring - time of decline of the testes. A change in postnatal development caused by this substance was not observed. The male offspring were killed and examined in groups of 17 animals at three different dates (65th, 100th and 130th day of life, respectively). In order to evaluate the function of the testes and the fertility, testes and epididymides were weighed, the number of spermatids and sperms was counted and the morphology of sperms and histological enzyme chemistry were checked. In addition, prior to the examination, some animals of each group were mated with untreated female rats to create a F2 generation. In those cases where the number of spermatides significantly differed, it was lower in the test groups than in the control group. The sperms morphology data of the test groups were worse in the same manner. The random testing of the enzyme activity of the Leydig cells showed in some cases far worse results in the test groups than in the control group. The data from the F2 generation were unconspicuous. Spironolactone adversely affects the function of the testes. (Read More)
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