Authors:
Sara Esther Diomande, Sara Esther Diomande, Stéphanie Chamot, Stéphanie Chamot, Vera Antolinos, Florian Vasai, Florian Vasai, Marie-Hélène Guinebretière, Marie-Hélène Guinebretière, Isabelle Bornard, Christophe Nguyen-The, Christophe Nguyen-T (...)
Sara Esther Diomande, Sara Esther Diomande, Stéphanie Chamot, Stéphanie Chamot, Vera Antolinos, Florian Vasai, Florian Vasai, Marie-Hélène Guinebretière, Marie-Hélène Guinebretière, Isabelle Bornard, Christophe Nguyen-The, Christophe Nguyen-The, Véronique Broussolle, Véronique Broussolle, Julien Brillard, Julien Brillard
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Abstract:
ABSTRACT The different strains of Bacillus cereus can grow at temperatures covering a very diverse range. Some B. cereus strains can grow in chilled food and consequently cause food poisoning. We have identified a new sensor/regulator mechanism involved in low-temperature B. cereus growth. Construction of (...)
ABSTRACT The different strains of Bacillus cereus can grow at temperatures covering a very diverse range. Some B. cereus strains can grow in chilled food and consequently cause food poisoning. We have identified a new sensor/regulator mechanism involved in low-temperature B. cereus growth. Construction of a mutant of this two-component system enabled us to show that this system, called CasKR, is required for growth at the minimal temperature ( T min ). CasKR was also involved in optimal cold growth above T min and in cell survival below T min . Microscopic observation showed that CasKR plays a key role in cell shape during cold growth. Introducing the casKR genes in a Δ casKR mutant restored its ability to grow at T min . Although it was first identified in the ATCC 14579 model strain, this mechanism has been conserved in most strains of the B. cereus group. We show that the role of CasKR in cold growth is similar in other B. cereus sensu lato strains with different growth temperature ranges, including psychrotolerant strains.
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