Authors:
Victor M. Corman, Marcel A. Müller, Ulrich Costabel, Jörg Timm, Tabea Binger, Bernhard Meyer, Petra Kreher, Erik Lattwein, Monika Eschbach-Bludau, Andreas Nitsche, Tobias Bleicker, Olfert Landt, Brunhilde Schweiger, Jan Felix Drexler, Albert D. M. (...)
Victor M. Corman, Marcel A. Müller, Ulrich Costabel, Jörg Timm, Tabea Binger, Bernhard Meyer, Petra Kreher, Erik Lattwein, Monika Eschbach-Bludau, Andreas Nitsche, Tobias Bleicker, Olfert Landt, Brunhilde Schweiger, Jan Felix Drexler, Albert D. M. E. Osterhaus, Bart L. Haagmans, Ulf Dittmer, F. Bonin, Thorsten Wolff, Christian Drosten
(Read More)
Abstract:
We present a rigorously validated and highly sensitive confirmatory real-time RT-PCR assay (1A assay) that can be used in combination with the previously reported upE assay. Two additional RT-PCR assays for sequencing are described, targeting the RdRp gene (RdRpSeq assay) and N gene (NSeq assay), where an insertion/deletion polymorphism might exist among different hCoV-EMC strains. Finally, a simplified and biologically safe protocol for detection of antibody response by immunofluorescence microscopy was developed using convalescent pat (...)
We present a rigorously validated and highly sensitive confirmatory real-time RT-PCR assay (1A assay) that can be used in combination with the previously reported upE assay. Two additional RT-PCR assays for sequencing are described, targeting the RdRp gene (RdRpSeq assay) and N gene (NSeq assay), where an insertion/deletion polymorphism might exist among different hCoV-EMC strains. Finally, a simplified and biologically safe protocol for detection of antibody response by immunofluorescence microscopy was developed using convalescent patient serum.
(Read More)