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2020 • V.4 - Direct wastewater RNA capture and purification via the "Sewage, Salt, Silica and SARS-CoV-2 (4S)" method

Authors: Oscar N Whitney, Basem Al-Shayeb, Alex Crits-Cristoph, Mira Chaplin, Vinson Fan, Hannah Greenwald, Adrian Hinkle, Rose Kantor, Lauren Kennedy, Anna Maurer, Robert Tjian, UC Berkeley Wastewater-based epidemiology consortium, Kara L Nelson

Abstract:

This protocol describes the procedure of the "4S" (Sewage, Salt, Silica and SARS-CoV-2) method for SARS-CoV-2 RNA extraction from wastewater. Offering a highly efficient, modular and economical alternative to existing wastewater RNA purification methods, this procedure lowers the barrier to entry for SARS-CoV-2 wastewater-based epidemiology. This procedure is intended to be carried out in a BSL2+ laboratory space, with precautions when handling raw wastewater samples.

External links: OpenAIRE

Works in BIP! associated with the specified OpenAIRE research product

V.4 - Direct wastewater RNA capture and purification via the "Sewage, Salt, Silica (...)

Oscar N Whitney, Basem Al-Shayeb, Alex Crits-Cristoph, Mira Chaplin, Vinson Fan, Hannah Greenwald, A (...)

N/A · 2020

Chromatography | Pulp and paper industry | Waste management |

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