Abstract: Transcriptional expression of DUX4 target genes and the PCDHγ cluster in SMCHD1 KD 4qB cell lines. RNA expression analysis of (A) the DUX4 target genes ZSCAN4 and LEUTX in primary myotubes and the PCDHγ cluster isoform members in primary (B) myoblast and (C) myotube cells after SMCHD1 shRNA KD or control shRNA KD targeting luciferase and GFP. Results represent qRT-PCR analysis of the indicated gene after normalization to the internal control gene GUS1. For each gene, the value of expression in control shRNA samples was then arbitrarily set to...
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