Authors: Narendra Bharathy, Berlow, Noah, Wang, Eric, Jinu Abraham, Settelmeyer, Teagan, Hooper, Jody, Svalina, Matthew, Bajwa, Zia, Goros, Martin, Hernandez, Brian, Wolff, Johannes, Ranadip Pal, Davies, Angela, Arya Ashok, Bushby, Darnell, Mancini, Maria, Noakes, Christopher, Goodwin, Neal, Ordentlich, Peter, Keck, James, Hawkins, Douglas, Rudzinski, Erin, Atiya Mansoor, Perkins, Theodore, Vakoc, Michalek, Joel, Keller, Charles

Venue: Figshare

Type: Dataset

Abstract: Figure S3. CRISPR/Cas9 mediated HDACs inhibition and evaluation of tumor cell growth inhibition in eRMS. (a-e) CRISPR/Cas9 screen for viability of selected HDACs (HDAC1-2 & HDAC10-11) excision by CRISPR in murine eRMS. (f) Q-PCR of murine eRMS U57810 for the expression of myogenic markers of differentiation in vitro. Data normalized to GAPDH expression. Gene expression was quantified using 2â ^dCt method. Myogenin (MYOG), Myoblast determination protein 1 (MyoD) and Myoglobin (Mb). (TIF 2070 kb)

DOI: 10.6084/m9.figshare.8163938.v1 (Found 2 versions)