Authors: Barchi, Lorenzo, Acquadro, Alberto, Alonso, David, Aprea, Giuseppe, Bassolino, Laura, Demurtas, Olivia, Ferrante, Paola, Gramazio, Pietro, Mini, Portis, Ezio, Scaglione, Davide, Toppino, Vilanova, Santiago, Díez, María José, Rotino, Giuseppe Leonardo, Lanteri, Sergio, Prohens, Jaime, Giuliano, Giovanni
Venue: Frontiers in Plant Science
Type: Publication
Abstract: [EN] Single primer enrichment technology (SPET) is a new, robust, and customizable solution for targeted genotyping. Unlike genotyping by sequencing (GBS), and like DNA chips, SPET is a targeted genotyping technology, relying on the sequencing of a region flanking a primer. Its reliance on single primers, rather than on primer pairs, greatly simplifies panel design, and allows higher levels of multiplexing than PCR-based genotyping. Thanks to the sequencing of the regions surrounding the target SNP, SPET allows the discovery of thousands of clo...
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Topics: 
Genetics
Computational biology
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