Abstract: Shiga toxin-producingEscherichia coli(STEC) have been linked to food-borne disease outbreaks. As PCR is routinely used to screen foods for STEC, it is important that factors leading to inconsistent detection of STEC by PCR are understood. This study used whole genome sequencing (WGS) to investigate causes of inconsistent PCR detection ofstx1,stx2, and serogroup-specific genes. Fifty strains isolated from Alberta feedlot cattle from three different studies were selected with inconsistent or consistent detection ofstxand serogroup by PCR. All iso...
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Topics: 
Microbiology
Virology
Genetics