Authors: Jing, Xiao, Hongyan, Jia, Liping, Pan, Zihui, Li, Lingna, Lv, Boping, Du, Lanyue, Zhang, Fengjiao, Yinxia, Huang, Tingming, Cao, Qi, Sun, Rongrong, Wei, Aiying, Xing, Zongde
Venue: Infection, Genetics and Evolution
Type: Publication
Abstract: Despite technical advances in introducing genomic deletions and modulating gene expression, direct inactivation of essential genes in mycobacteria remains difficult. In this study, we described clustered regularly interspaced short palindromic repeat interference (CRISPRi) technology to repress the expression of sepF (MSMEG_4219) based on nuclease-deficient CRISPR-associated protein 9 (Cas9) and small guide RNA (sgRNA) specific to the target sequence in Mycobacterium smegmatis. Using this CRISPRi approach, we achieved the repression of sepF by ...
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Topics: 
Cell biology
Computational biology
Genetics
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