Abstract: In this chapter, we described a bottom-up approach for the reconstitution of three-dimensional cellular multilayered tissue for the application of tissue engineering and drug testing models. Based on the technology of polymer chemistry, we performed nanofilm coating with extracellular matrix to living cells by layer-by-layer assembly to induce cell–cell interaction for the adhesion. Using fibronectin–gelatin nanofilms, various cellular multilayers including fibroblast tissues, liver tissues, and blood vessel structures were fabricated. Espe...
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Topics: 
Biomedical engineering
Nanotechnology