1998 •
Improved DNA extraction from ancient bones using silica-based spin columns
Authors: Dongya Y. Yang, Barry Eng, John S. Waye, J. Christopher Dudar, Shelley R. Saunders
Venue: American Journal of Physical Anthropology
Type: Publication
Abstract: We describe a simple method for extracting polymerase chain reaction-amplifiable DNA from ancient bones without the use of organic solvents. Bone powders are digested with proteinase K, and the DNA is purified directly using silica-based spin columns (QIAquick3, QIAGEN). The efficiency of this protocol is demonstrated using human bone samples ranging in age from 15 to 5,000 years old.
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